Generator

Part:BBa_I766665:Design

Designed by: alexander ng   Group: iGEM07_UCSF   (2007-08-16)


YopJ-EE under strong constitutive promotor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1871
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1871
    Illegal NotI site found at 3311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2527
    Illegal BamHI site found at 2368
    Illegal BamHI site found at 3294
    Illegal XhoI site found at 1511
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1871
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1871
    Illegal NgoMIV site found at 224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 874
    Illegal BsaI.rc site found at 2296
    Illegal SapI.rc site found at 2320


Design Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonucleases AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.


Source

composite part

References