![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_I766665:Design
Designed by: alexander ng Group: iGEM07_UCSF (2007-08-16)
YopJ-EE under strong constitutive promotor
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1871
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1871
Illegal NotI site found at 3311 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2527
Illegal BamHI site found at 2368
Illegal BamHI site found at 3294
Illegal XhoI site found at 1511 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1871
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1871
Illegal NgoMIV site found at 224 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 874
Illegal BsaI.rc site found at 2296
Illegal SapI.rc site found at 2320
Design Notes
Device was created using a method of combinatorial cloning using the type IIs restriction endonucleases AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.
Source
composite part